Podophyllum Toxin

Podophyllum Toxin USP

Written by

Reviewed by

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Gaurav Sood

Partner - HimPharm

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Dr B M Sood

Chairman - HimPharm

Podophyllum Toxin Manufactured by HimPharm

Category: Phytochemical
Trade Names: Podophyllotoxin
Routes of Administration: Topical
ATC Code: Not Applicable


  • CAS Number: 518-28-5
  • PubChem CID: 104819
  • ChemSpider ID: 94618

Chemical and Physical Data

  • Molecular Formula C22H22O8
  • Molar Mass 414.41 g/mol

SMILES: COC1=CC2=C(C(=C1)[C@@H]3[C@@H]2[C@H]4C@HC5=C3C(=C(C=C5)O)O)OCO

InChI: InChI=1S/C22H22O8/c1-25-16-7-11(8-17(26-2)21(16)27-3)15-12-5-9(23)6-13-18(12)28-22(24)19(15)20(13)29-14-4-10(15)30-14/h5-8,14,19-20,23-24H,4H2,1-3H3/t14-,19-,20+/m0/s1


Podophyllum Toxin

Podophyllum Toxin USP, a non-alkaloid toxin lignan of the lignan family, stands as a pivotal component in modern medicine. Derived from the roots and rhizomes of Podophyllum species, this pharmacological marvel has garnered significant attention for its therapeutic properties and diverse applications in healthcare. 

Renowned for its efficacy in treating genital warts and molluscum contagiosum, Podophyllum Toxin USP serves as a beacon of hope for patients grappling with dermatological ailments

Podophyllum Toxin

Impact of Podophyllumon Pharmaceutical, Drug, and Natural Products Industry

The advent of Podophyllum Toxin USP has heralded a new era in the pharmaceutical, drug, and natural products industry. Its emergence as a potent anti-wart agent has revolutionized the treatment landscape for dermatological conditions, offering patients a safe and effective remedy for their ailments. 

Moreover, Podophyllum Toxin USP serves as the pharmacological precursor for the esteemed anti-cancer drug etoposide, underscoring its pivotal role in oncological therapeutics.

In the realm of natural products, Podophyllum Toxin USP has emerged as a beacon of innovation, inspiring researchers and manufacturers to explore its therapeutic potential across diverse therapeutic domains. 

From topical treatments for skin disorders to novel chemotherapeutic agents in oncology, the versatility of Podophyllum Toxin USP continues to captivate the imagination of healthcare professionals and researchers alike.

Understanding Podophyllum Toxin

What is Podophyllum Toxin USP?

Podophyllum Toxin USP, a non-alkaloid toxin lignin, is a key component derived from the roots and rhizomes of Podophyllum species. Podophyllin stands as a pivotal member of the lignan family, renowned for its pharmacological significance and therapeutic properties. This toxin plays a vital role in the treatment of various medical conditions, particularly in dermatology and oncology.

Renowned for its efficacy and safety, Podophyllum Toxin USP has garnered attention in both traditional and modern medicine spheres. Its molecular structure, consisting of carbon, hydrogen, and oxygen atoms, underscores its organic composition and intricate pharmacological properties.


Applications of Podophyllum Toxin USP

Podophyllum Toxin USP finds extensive application in the pharmaceutical and healthcare industries. Its primary use lies in the treatment of skin conditions, notably genital warts and molluscum contagiosum. Through its mitotic inhibition mechanism, Podophyllum Toxin USP targets rapidly dividing cells, making it an effective therapeutic agent against various proliferative disorders.

Additionally, Podophyllum Toxin USP serves as a precursor for the synthesis of etoposide, a potent anti-cancer drug used in chemotherapy regimens. Its cytotoxic properties make it indispensable in oncology, where it combats malignant cells and inhibits tumor growth.

Physical Characteristics of Podophyllum Toxin USP

Podophyllum Toxin USP typically manifests as a gel or solution, optimized for topical application. Its consistency and viscosity ensure optimal adherence to affected areas, facilitating targeted treatment and maximum efficacy. In its refined form, Podophyllum Toxin USP exhibits a clear and transparent appearance, indicative of its purity and pharmaceutical grade quality.

Source and Extraction of Podophyllum Toxin USP

The extraction of Podophyllum Toxin USP involves meticulous processes to isolate the active compound from Podophyllum roots and rhizomes. This botanical source undergoes solvent-based extraction techniques, followed by purification and concentration steps to obtain a refined product with high potency and minimal impurities.

Importance and Versatility of Podophyllum Toxin USP

Podophyllum Toxin USP holds significance as both a therapeutic agent and a precursor for anti-cancer drugs. Its multifaceted pharmacological properties make it a valuable asset in modern medicine, offering solutions for a diverse range of medical conditions. Moreover, its botanical origin underscores its appeal in complementary and alternative medicine practices, catering to holistic wellness approaches.

Diverse Uses of Podophyllum Toxin USP in Various Industries

Beyond its medical applications, Podophyllum Toxin USP finds utility in other industries such as cosmetics and herbal products. Its natural origins and pharmacological properties make it a desirable ingredient in skincare formulations and herbal remedies. The versatility of Podophyllum Toxin USP extends beyond conventional medicine, catering to the evolving needs of diverse consumer markets.

The History of Podophyllum Toxin USP

Discovery and Origin of Podophyllum Toxin USP

The history of Podophyllum Toxin USP traces back to its discovery in the late 19th century. The roots and rhizomes of Podophyllum species, particularly Podophyllum peltatum and Sinopodophyllum hexandrum, have long been recognized for their medicinal properties by indigenous cultures across the globe. 

However, it was Valerian Podwyssotzki, a Polish-Russian pharmacologist, who first isolated Podophyllum Toxin USP in its pure form in 1880.

Contributions of Valerian Podwyssotzki to Podophyllum Toxin USP

Valerian Podwyssotzki’s pioneering work at the University of Dorpat (now Tartu, Estonia) laid the foundation for the modern understanding of Podophyllum Toxin USP’s pharmacological properties. His meticulous research and experimentation paved the way for its pharmacological applications in dermatology and oncology, marking a significant milestone in medical science.

Evolution and Milestones in the Development of Podophyllum Toxin USP

Since its discovery, Podophyllum Toxin USP has undergone extensive research and development, leading to advancements in its formulation and therapeutic applications. The identification of its cytotoxic properties opened new avenues in cancer treatment, culminating in the synthesis of etoposide, a cornerstone in modern chemotherapy regimens. 

Over the years, Podophyllum Toxin USP has emerged as a vital component in the armamentarium against various dermatological and oncological disorders, contributing to improved patient outcomes and quality of life

Exploring Podophyllum Toxin USP and its Use in Pharmaceuticals and Health Industry

Therapeutic Uses and Properties of Podophyllum Toxin USP

Podophyllum Toxin USP boasts a myriad of therapeutic uses, primarily centered around its cytotoxic and anti-proliferative properties. In dermatology, it serves as a frontline treatment for genital warts and molluscum contagiosum, exerting its effects through cell cycle arrest and apoptosis induction. 

Its selective targeting of rapidly dividing cells makes it an effective chemotherapeutic agent against various cancers, including leukemia, lymphoma, and solid tumors.

Additional Properties and Supplementary Uses of Podophyllum Toxin USP

Beyond its primary indications, Podophyllum Toxin USP exhibits diverse pharmacological properties with potential applications in complementary and alternative medicine. Its anti-inflammatory and antioxidant effects make it a promising candidate for skincare formulations and herbal remedies. 

Moreover, ongoing research explores its neuroprotective and immunomodulatory properties, hinting at broader therapeutic potential in neurological and autoimmune disorders.

The Raw Material from Which Podophyllum Toxin USP is Obtained

Raw Material Overview

The raw material for Podophyllum Toxin USP primarily originates from the roots and rhizomes of Podophyllum species, notably Podophyllum peltatum and Sinopodophyllum hexandrum. These plants, commonly known as mayapples or mandrakes, thrive in diverse ecological niches, including temperate forests and alpine regions. The botanical richness of these species provides a sustainable source of raw material for pharmaceutical extraction.

What is the Raw Material?

The raw material for Podophyllum Toxin USP consists of the underground structures of Podophyllum species, encompassing the roots and rhizomes. These plant parts harbor a reservoir of bioactive compounds, including podophyllotoxin, the primary constituent of Podophyllum Toxin USP.

Where is it Found?

Podophyllum species are distributed across various geographical regions, with Podophyllum peltatum predominantly found in North America and Sinopodophyllum hexandrum endemic to the Himalayan region. The unique ecological habitats of these plants influence the composition and potency of the raw material.

What is its Botanical Name?

The botanical names of the primary sources of Podophyllum Toxin USP are Podophyllum peltatum and Sinopodophyllum hexandrum. These taxonomic designations underscore the botanical classification and phylogenetic relationships of these plant species.

Alternative Sources of Raw Material for Extracting Podophyllum Toxin USP

While Podophyllum peltatum and Sinopodophyllum hexandrum serve as the principal botanical sources for Podophyllum Toxin USP, alternative plant species have also been explored for their potential as raw material. Species such as Podophyllum emodi and Podophyllum pleianthum contain varying concentrations of podophyllotoxin, offering alternative avenues for pharmaceutical extraction.

HimPharm Manufacturing Standards

HimPharm’s Role in Podophyllum Toxin USP Production

HimPharm stands as a beacon of excellence in the production of Podophyllum Toxin USP, epitomizing unwavering commitment to quality and innovation. With a legacy spanning over four decades, HimPharm has carved a niche for itself in the pharmaceutical landscape, distinguished by its adherence to stringent manufacturing standards and relentless pursuit of excellence.

At the core of HimPharm’s manufacturing ethos lies a steadfast commitment to harnessing nature’s bounty responsibly and ethically. Through sustainable sourcing practices and meticulous extraction techniques, HimPharm ensures the preservation of biodiversity while extracting the potent bioactive compounds inherent in Podophyllum species.

Upholding International Quality Standards

HimPharm’s unwavering dedication to quality extends beyond regulatory compliance, encompassing every facet of the manufacturing process. From raw material procurement to product formulation and quality control, HimPharm adheres to international quality standards with unwavering diligence.

The state-of-the-art manufacturing facilities at HimPharm are equipped with cutting-edge technology and robust quality control mechanisms, ensuring the integrity and potency of Podophyllum Toxin USP at every stage of production. Rigorous testing protocols and validation procedures validate product efficacy and safety, instilling confidence in healthcare professionals and end-users alike.

HimPharm’s Commitment to Fulfilling Bulk Requirements for Podophyllum Toxin USP

HimPharm recognizes the diverse needs of its clientele, ranging from pharmaceutical giants to niche market segments. With scalable production capabilities and flexible manufacturing processes, HimPharm caters to bulk requirements for Podophyllum Toxin USP with unparalleled efficiency and reliability.

Whether sourcing ingredients for large-scale pharmaceutical formulations or formulating specialty products for niche markets, HimPharm’s agility and responsiveness ensure timely delivery and consistent quality across diverse product portfolios. 

As a trusted partner in pharmaceutical manufacturing, HimPharm empowers its clients to realize their vision while upholding the highest standards of quality and integrity.

Podophyllum Toxin USP Production Processes

Step-by-Step Manufacturing Process

Details of the Step-by-Step Manufacturing Process of Podophyllum Toxin USP

The manufacturing journey of Podophyllum Toxin USP unfolds through a series of meticulously orchestrated steps, each crucial in ensuring the purity, potency, and safety of the final product. HimPharm’s state-of-the-art facilities and experienced personnel adhere to stringent quality control measures, guaranteeing pharmaceutical-grade Podophyllum Toxin USP of the highest caliber.

Step 1: Raw Material Preparation

The process commences with the careful selection and procurement of high-quality roots and rhizomes from Podophyllum species. These botanical sources undergo rigorous quality assessment to ascertain their potency and purity before being subjected to extraction.

Step 2: Extraction

The selected roots and rhizomes undergo extraction using solvent-based techniques, aimed at isolating the bioactive compounds, primarily podophyllotoxin. The choice of solvent and extraction parameters is optimized to maximize yield and minimize degradation, ensuring the preservation of the compound’s pharmacological efficacy.

Step 3: Purification

The extracted material undergoes purification processes to remove impurities and unwanted substances, further refining the podophyllotoxin extract. Chromatographic techniques such as column chromatography or high-performance liquid chromatography (HPLC) are employed to separate the target compound from other components, ensuring a high degree of purity.

Step 4: Concentration

The purified extract is concentrated to enhance the potency and efficacy of the active compound. Evaporation or solvent removal techniques are employed to achieve the desired concentration levels, optimizing the therapeutic effects of Podophyllum Toxin USP in pharmaceutical formulations.

Step 5: Quality Control Testing

Rigorous quality control testing is conducted throughout the manufacturing process to assess the purity, potency, and safety of Podophyllum Toxin USP. Analytical techniques such as spectroscopy, chromatography, and microbiological assays are employed to validate product quality and compliance with regulatory standards.

Step 6: Packaging and Distribution

Upon successful completion of quality control testing, the finalized product undergoes packaging and labeling procedures before distribution to pharmaceutical companies, healthcare institutions, and bulk consumers worldwide. Stringent packaging standards ensure product integrity and tamper-proof security, safeguarding the potency and efficacy of Podophyllum Toxin USP during transit and storage.

Testing in Podophyllum Toxin USP Manufacturing

Ensuring Quality and Safety

Quality control serves as the cornerstone of Podophyllum Toxin USP manufacturing at HimPharm, underpinning the integrity, potency, and safety of the final product. Through a comprehensive array of testing procedures and validation protocols, HimPharm upholds the highest standards of quality assurance, instilling confidence in healthcare professionals and end-users alike.

Identity Testing:

Verification of the authenticity and botanical identity of the raw material is paramount in ensuring product integrity. Macroscopic and microscopic examination techniques are employed to confirm the presence of characteristic morphological features consistent with Podophyllum species. By establishing botanical identity, HimPharm mitigates the risk of adulteration and ensures the purity of Podophyllum Toxin USP.

Purity Analysis:

Assessment of the purity of the extracted compound is essential to eliminate contaminants and impurities that may compromise product quality. Chemical analysis techniques, including thin-layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS), enable quantification of podophyllotoxin and identification of potential impurities. HimPharm’s stringent purity standards guarantee the absence of deleterious substances, safeguarding patient safety.

Potency Assay:

Determination of the concentration of active compounds, particularly podophyllotoxin, is critical in ensuring therapeutic efficacy and consistency across batches. High-performance liquid chromatography (HPLC) and spectrophotometric methods are utilized to quantify podophyllotoxin content with precision and accuracy. 

HimPharm’s commitment to potency assay testing underscores its dedication to delivering products of unparalleled efficacy and reliability.

Microbiological Testing:

Screening for microbial contaminants is imperative to maintain product safety and compliance with regulatory standards. Microbiological assays, including total viable count and microbial limit tests, detect the presence of bacteria, fungi, and other microorganisms that may pose health risks. HimPharm’s stringent microbiological testing protocols mitigate the risk of microbial contamination, ensuring product purity and safety.

Stability Testing:

Evaluation of the stability of Podophyllum Toxin USP under various storage conditions is essential to determine shelf-life and product integrity over time. Accelerated stability studies and real-time monitoring assess the impact of temperature, humidity, and light exposure on product stability and degradation kinetics. 

HimPharm’s comprehensive stability testing protocols guarantee product efficacy and reliability throughout its shelf-life.

Incorporating these testing procedures into the manufacturing workflow, HimPharm maintains rigorous quality control standards, ensuring the purity, potency, and safety of Podophyllum Toxin USP at every stage of production.

Navigating Production Bottlenecks in Podophyllum Toxin USP Manufacturing

In the dynamic landscape of pharmaceutical manufacturing, production bottlenecks pose inherent challenges that necessitate innovative solutions and proactive management strategies. HimPharm remains vigilant in identifying and addressing potential bottlenecks, leveraging its expertise and infrastructure to streamline production processes and enhance operational efficiency.

Raw Material Sourcing:

Ensuring a consistent and reliable supply of high-quality raw materials is paramount to sustaining manufacturing operations. HimPharm maintains robust relationships with trusted suppliers and implements stringent quality control measures to verify the authenticity and potency of botanical sources. 

By securing a stable raw material supply chain, HimPharm mitigates the risk of production delays and maintains product integrity.

Process Optimization:

Streamlining manufacturing processes is essential to enhance efficiency and throughput without compromising product quality. HimPharm continually evaluates and refines production workflows, employing lean manufacturing principles and advanced process optimisation techniques. 

By optimising resource allocation and minimising waste, HimPharm maximises productivity and reduces production cycle times.

Regulatory Compliance:

Navigating complex regulatory requirements is integral to ensuring compliance with international quality standards and Good Manufacturing Practices (GMP). HimPharm maintains robust regulatory oversight and invests in continuous training and education to keep abreast of evolving regulatory frameworks. 

By adhering to stringent regulatory guidelines, HimPharm demonstrates its commitment to product safety and quality assurance.

Supply Chain Logistics:

Managing logistics and distribution channels is crucial to minimising lead times and ensuring timely delivery of finished products to customers. HimPharm leverages advanced inventory management systems and strategic partnerships with logistics providers to optimize supply chain efficiency. 

By implementing just-in-time inventory practices and agile distribution networks, HimPharm reduces inventory holding costs and enhances customer responsiveness.

HimPharm addresses production bottlenecks through strategic planning, continuous process improvement, and investment in advanced manufacturing technologies. By fostering a culture of innovation and excellence, HimPharm remains at the forefront of pharmaceutical manufacturing, delivering high-quality Podophyllum Toxin USP to meet the evolving needs of healthcare professionals and patients worldwide.

HimPharm’s Manufacturing Advantage

HimPharm’s manufacturing advantage lies in its unwavering commitment to excellence and innovation, coupled with its robust infrastructure and seasoned workforce. 

By embracing cutting-edge technologies and adhering to stringent quality standards, HimPharm stands poised to meet the evolving demands of the pharmaceutical industry, driving progress and innovation in pharmaceutical manufacturing.


Leveraging HimPharm’s Commitment to Quality

In conclusion, the journey of Podophyllum Toxin USP encapsulates a narrative of scientific discovery, therapeutic innovation, and manufacturing excellence. As a key player in the pharmaceutical landscape, HimPharm has pioneered the production of Podophyllum Toxin USP, setting new benchmarks for quality and reliability in pharmaceutical manufacturing.

We have looked into the multifaceted dimensions of Podophyllum Toxin USP, exploring its origins, therapeutic properties, and industrial applications. From its botanical roots to its pivotal role in the treatment of genital warts and molluscum contagiosum, Podophyllum Toxin USP emerges as a cornerstone of modern medicine, offering hope and healing to patients worldwide.

HimPharm’s unwavering commitment to quality and innovation underscores its role as a trusted partner in the pharmaceutical industry. With a legacy spanning over four decades, HimPharm has remained at the forefront of pharmaceutical manufacturing, leveraging its expertise and infrastructure to deliver high-quality Podophyllum Toxin USP to global markets.

By upholding international quality standards, navigating production bottlenecks, and embracing technological advancements, HimPharm continues to redefine the contours of pharmaceutical excellence. As the healthcare landscape evolves, HimPharm remains steadfast in its commitment to advancing the frontiers of pharmaceutical innovation, delivering life-saving therapies and enhancing patient care.

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